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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Cell Physiol. 2017 Oct 4;233(4):3230–3243. doi: 10.1002/jcp.26166

Figure 7.

Figure 7

The actions of NPP1 contribute towards the inhibitory effects of ATP and UTP on VSMC calcification and bone mineralization. The inhibitory effects of (a) ATP and (b) UTP on calcification were 10-fold less potent in Enpp1−/− VSMCs. In Enpp1−/− osteoblasts (c) ATP and (d) UTP were 10-fold and 100-fold less potent, respectively, at blocking bone mineralization. Values are mean ± SEM (n = 6), *p < 0.05, **p < 0.01, ***p < 0.001. Comparison of Enpp1+/+ to Enpp1−/−: #p< 0.05, ###p < 0.001. (e) Representative whole well scans (unstained) and phase contrast microscopy images (alizarin red stained) showing the effects of ATP on bone mineralization in Enpp1+/+ and Enpp1−/− osteoblasts. Scale bars: whole well scans = 0.5 cm, microscopy images = 200 μm