Fig. 1.

Repetitive intradermal bleomycin induces inflammation and fibrosis in multiple organs. Wild-type mice were injected with saline or bleomycin (6 U/kg body wt; one time/day; 5 days/wk) via the id route for 4 wk. A: at the end of 4 wk, skin tissue of 6 mm in diameter was collected from the injected site, and 5-μm sections of paraffin-embedded skin tissues were stained with Masson’s trichrome to indicate collagen deposition (blue) in skin. All images were collected at the original magnification of ×10 (scale bars, 200 µm). B: 5-μm sections of paraffin-embedded lungs tissues were stained with hematoxylin and eosin (H&E) to indicate inflammation in the subpleural (top) and peribronchial (bottom) regions. All images were collected at an original magnification of ×40 (scale bars, 100 µm). C: 5-μm sections of paraffin-embedded lungs tissues were stained with Masson’s trichrome to indicate collagen deposition (blue) in subpleural (top) and peribronchial (bottom) regions. All images were collected at an original magnification of ×40 (scale bars, 100 µm). Data are representative of >4 independent experiments that produced similar results.