Fig. 5.

The lack of IL-4Rα-driven signaling is sufficient to attenuate pulmonary fibrosis. Wild-type and IL-4Rα knockout mice were treated with bleomycin via the id route for 4 wk. A: at the end of 4 wk, paraffin-embedded lung tissue sections were stained with Masson’s trichrome to indicate collagen deposition (blue) in subpleural (top) and peribronchial (bottom) regions. All images were collected at an original magnification of ×5 (scale bars, 250 µm). Dashed boxes in the ×5 images indicate the areas highlighted in the ×40 magnification (scale bars, 50 µm) and presented at the left corner of original images. B: lung lysates were blotted with anti-Col1A1, and equal loading was confirmed by GAPDH staining. C and D: resistance (C) and compliance (D) of the lungs were measured using a FlexiVent. Data are presented as means ± SE (n = 5–6 mice/group), and unpaired Student’s t-test was used to measure the significant difference between the groups, *P < 0.05, **P < 0.005, and ***P < 0.000.5. All experiments were repeated 2 times with similar results.