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A: detection of Ca2+ sparks in sham-operated (Sham), ovariectomized (OVx), and OVx + 17β-estradiol (OVx + E) Fluo-4-loaded myocytes using line scanning confocal microscopy. B: mean spark frequency was higher in OVx myocytes compared with Sham and OVx + E myocytes in the presence and absence of isoprenaline (ISO). Sham: n = 55/5, OVx: n = 36/3, and OVx + E: n = 40/4. **P < 0.01; ***P < 0.001.
