Relative amounts of Cav1.2 and Cavβ2 mRNA after ovariectomy (OVx). A: relative Cav1.2 mRNA expression (normalized to β-actin mRNA) was unaltered between sham-operated (Sham), OVx, and OVx + 17β-estradiol replacement (OVx + E) myocytes. Sham: n = 7, OVx: n = 7, and OVx + E: n = 6. B: relative Cav β2 mRNA expression (normalized to β-actin mRNA) did not change after OVx and OVx + E compared with Sham treatment. Sham: n = 7, OVx: n = 7, and OVx + E: n = 6.