Fig. 4.

EFS-evoked release of purines upon pharmacological inhibition or stimulation of nitric oxide signaling in wild-type (WT) and W/W^v mouse colon. A: chromatograms of tissue superfusates collected during EFS of WT (left) and W/W^v (right) mouse colon in the absence of drugs (control) or in the presence of l-NNA (100 μM for 60 min) or SNAP (1 μM for 5 min). Inhibition of NO with l-NNA further increased the EFS-evoked release of purines in WT and W/W^v mice whereas the NO donor, SNAP, reduced the EFS-evoked release of purines. LU, luminescence units. B: averaged data (means ± SE) summarizing the EFS-evoked release of NAD⁺/ADPR, ATP, and total purines (i.e., ATP+ADP+NAD⁺/ADPR+AMP+ADO) in the WT and W/W^v mouse colon in the absence (Ctr, control) and presence of l-NNA and SNAP. Release in femtomoles per milligram of tissue (fmol/mg) is calculated as evoked release minus spontaneous release. Significant difference between groups is identified with horizontal bars (*P < 0.05, **P < 0.01, Student’s t-test). Number of experiments is in parentheses.