Fig. 3.

Effect of prevailing thiamin level (DEF and OS) on expression of the mitochondrial TPP (MTPP) protein in PAC 266-6 (A) and mouse primary PAC (B). Protein (50 μg) of freshly isolated mitochondria from the PAC 266-6 maintained under thiamin-DEF and -OS conditions (A) and from primary PAC isolated from thiamin-DEF and -OS mice (B) were separated by SDS-PAGE and probed with anti-MTPPT antibodies as described in materials and methods. The relative protein expression of MTPPT was determined after normalization with pyruvate dehydrogenase (PDH). Inset: representative immunoblots. Data are means ± SE from 3 independent sets of experiments (**P < 0.01).