Table 1.
Antibodies | Catalog No. and Company | Western Blot Analysis | IF Staining |
---|---|---|---|
Rabbit anti-KLF5 | sc-22797, Santa Cruz Biotechnology | 1:1,000 diluted in 5% milk | 1:200 diluted in 10% horse serum |
Goat anti-KLF5 | AF3758, R&D | N/A | 1:100 diluted in 1% milk |
Rabbit anti-DSG2 | GTX102508, GeneTex | 1:1,000 diluted in 5% milk | 1:200 diluted in 10% horse serum |
Rabbit anti-DSG2 | AB150372, Abcam | N/A | 1:200 diluted in 1% milk |
Sheep anti-DSC2 | AF4688, R&D | 1:1,000 diluted in 5% milk | 1:100 diluted in 10% horse serum for immunocytochemistry 1:100 diluted in 1% milk for immunohistochemistry |
Mouse anti-GAPDH | MAB374, EMD Millipore | 1:5,000 diluted in 5% milk | N/A |
IF, immunofluorescence; KLF5, Krüppel-like factor 5; DSG, desmoglein-2; DSC2, desmocollin-2; N/A, not applicable.