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. 2017 Sep 1;313(5):G478–G491. doi: 10.1152/ajpgi.00172.2017

Table 1.

List of antibodies used for Western blot analysis and immunofluorescence staining

Antibodies Catalog No. and Company Western Blot Analysis IF Staining
Rabbit anti-KLF5 sc-22797, Santa Cruz Biotechnology 1:1,000 diluted in 5% milk 1:200 diluted in 10% horse serum
Goat anti-KLF5 AF3758, R&D N/A 1:100 diluted in 1% milk
Rabbit anti-DSG2 GTX102508, GeneTex 1:1,000 diluted in 5% milk 1:200 diluted in 10% horse serum
Rabbit anti-DSG2 AB150372, Abcam N/A 1:200 diluted in 1% milk
Sheep anti-DSC2 AF4688, R&D 1:1,000 diluted in 5% milk 1:100 diluted in 10% horse serum for immunocytochemistry
1:100 diluted in 1% milk for immunohistochemistry
Mouse anti-GAPDH MAB374, EMD Millipore 1:5,000 diluted in 5% milk N/A

IF, immunofluorescence; KLF5, Krüppel-like factor 5; DSG, desmoglein-2; DSC2, desmocollin-2; N/A, not applicable.