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. 2017 Sep 1;313(5):G478–G491. doi: 10.1152/ajpgi.00172.2017

Table 2.

List of primer sequences used for quantitative RT-PCR

Target Gene Primer Sequence
Cadherin 1 (Cdh1) Forward: 5′-CAGGTCTCCTCATGGCTTTGC-3′
Reverse: 5′-CTTCCGAAAAGAAGGCTGTCC-3′
Catenin-α1 (Ctnna1) Forward: 5′-AAGTCTGGAGATTAGGACTCTGG-3′
Reverse: 5′-ACGGCCTCTCTTTTTATTAGACG-3′
Catenin-β1 (Ctnnb1) Forward: 5′-ATGGAGCCGGACAGAAAAGC-3′
Reverse: 5′-CTTGCCACTCAGGGAAGGA-3′
Tight junction protein 1 (Tjp1) Forward: 5′-ACCACCAACCCGAGAAGAC-3′
Reverse: 5′-CAGGAGTCATGGACGCACA-3′
Occludin (Ocln) Forward: 5′-TTGAAAGTCCACCTCCTTACAGA-3′
Reverse: 5′-CCGGATAAAAAGAGTACGCTGG-3′
F11 receptor (F11r) Forward: 5′-TCTCTTCACGTCTATGATCCTGG-3′
Reverse: 5′-TTTGATGGACTCGTTCTCGGG-3′
Claudin 2 (Cldn2) Forward: 5′-CAACTGGTGGGCTACATCCTA-3′
Reverse: 5′-CCCTTGGAAAAGCCAACCG-3′
Claudin 4 (Cldn4) Forward: 5′-GTCCTGGGAATCTCCTTGGC-3′
Reverse: 5′-TCTGTGCCGTGACGATGTTG-3′