FIG. 2.

Vector maps of individual μbeacons (white X) and displacements (arrows) embedded in 0.1% type I collagen hydrogels before (A), during (B), and after (C) acoustic radiation force with 50% duty cycle. Vectors indicate interpolated direction and magnitude of displacement in the x–y plane, while heat maps indicate displacement in the z-plane. Results demonstrate that bead, and therefore hydrogel, displacement is not dominated by z-plane deflections, but rather is shared across all three axes of movement. Vector maps of individual μbeacons (white X) and displacements (arrows) embedded in 0.1% type I collagen hydrogels before (D), during (E), and after (F) acoustic radiation force with 100% duty cycle demonstrate that bead, and therefore hydrogel, displacement is greater in the z-direction with the higher duty cycle, indicating that increasing duty cycle increases hydrogel deformation.