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. 2017 Dec 7;103(2):246–255. doi: 10.3324/haematol.2017.177485

Figure 2.

Figure 2.

MiR-155 cooperates with Hoxa9 to induce leukemia in vivo. (A) Engraftment kinetics in peripheral blood (PB) of mice transplanted in three to four individual experiments with independently generated cell lines for each experiment of Hoxa9/ctrl (week 4 n=25; week 8 n=23; week 12 n=8), Hoxa9/miR-155 (week 4 n=24; week 8 n=20; week 12 n=5), Hoxa9/Meis1 (n=23), miR-155 (n=16) or a control vector (n=16) for each experiment. Engraftment was measured by flow cytometry analysis and data is shown as mean of green fluorescent protein (GFP)+ or yellow fluorescent protein (YFP)+ cells at 4, 8 and 12 weeks after transplantation. (B) Survival curves for cohorts of mice transplanted with independently generated Hoxa9/ctrl (n=25), Hoxa9/miR-155 (n=25) and Hoxa9/Meis1 (n=24) cells via a minimum of four individual transplantation experiments and transplantation of cells overexpressing miR-155 (n=16) or control (n=16) from three individual experiments. (C) Representative microscopic photographs of Wright-stained bone marrow (BM) cells (magnification 50×) are shown for mice that succumbed to leukemia. (D) Percentage of c-kit-expressing cells measured via flow cytometry in the BM cells of mice that developed leukemia after transplantation of Hoxa9/ctrl (n=8), Hoxa9/miR-155 (n=13) or Hoxa9/Meis1 (n=13) cells. See also Online Supplementary Figure S1 and Online Supplementary Figure S2.