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. 2017 Nov 23;103(2):266–277. doi: 10.3324/haematol.2017.170431

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Figure 5. — WT1-knockdown impairs TP53 DNA damage response in MOLT4 cells. (A) Relative expression of specific genes involved in the DNA-damage response. Analysis is performed in both Control (Ctrl) and WT1-KD cells after different times [3, 6 and 12 hours (h)] from 6 Gray of γ-radiation. Expression is calculated relative to untreated sh-Scramble MOLT4 cells (Ctrl 0 h) fixed as 1. (B) Western blot analysis of CDKN1A, BBC3, BAX and XIAP in Ctrl and WT1-KD MOLT4 cells after 1, 3, 6 and 12 h from γ-radiation. β–Actin is shown as loading control. Western blot quantification of CDKN1A, BBC3, BAX and XIAP proteins is reported on top of the gel. Values are normalized respect to the loading control. The numbers represent the relative intensity signal calculated respect to untreated Ctrl cells (fixed at 1). For CDKN1A and BBC3 proteins, that were not detected in untreated Ctrl cells, the relative intensity signal is calculated respected to 3 h and 1 h, respectively (Ctrl cells fixed at 1).