Figure 4.

Effects of oxygene saturation on hypoxanthine accumulation during refrigerated storage. (A) Human RBC were donated by healthy volunteers (n=4) prior to refrigerated storage in AS-3 under control (normoxic), hyperoxic (SO₂>95%), or hypoxic (SO₂ = 20%, 10%, 5% or <3%) conditions for up to 42 days. (B) Hypoxanthine concentration was determined in cells and supernatants, with significant decreases in the presence of hypoxia. All data points shown on the x axes were tested and interpolated with third order polynomial curves (not assuming linear evolution of hypoxanthine accumulation during storage) and medians ± ranges (n=4) are shown (dark blue lines and light blue areas, respectively).