Figure 2.

Co-administration of JAK2i and transfusion further improved extramedullary hematopoiesis. Administration of transfusion (indicated with TXF in the graphs) and JAK2i in a mouse model of β-thalassemia intermedia (NTDT-Hbb^th3/+) almost normalized spleen size compared to wild type (WT) values (A). In the same mice, a reduction in the proportion of nucleated erythroid cells in the spleen was detected by flow cytometry (B). When the administration of a JAK2i was combined with transfusion, suppression of erythropoiesis was more pronounced, as indicated by a decreased number of endogenous RBC (C). Splenomegaly was also improved in a mouse model of β-thalassemia major (TDT-C57-FLC^th3/th3) when JAK2i and transfusion were administered simultaneously (D). Analysis was performed using One-way ANOVA with Tukey multiple comparison adjustment or Two-way ANOVA with Sidak’s multiple comparison adjustment. Results represent mean ± SD, ****P<0.0001, ***P<0.001, **P<0.01.