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. 2018 Jan 31;8:1952. doi: 10.1038/s41598-018-20422-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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- DHA decreased cell viability of breast cancer cells (MDA-MB-231 and 4T1) but not normal mammary epithelial cells after 24 h of stimulation. MDA-MB-231 (A,B) and PBMC (C,D) were stimulated with different concentrations of AA or DHA for 24 h (lines with black circle) or 48 h (lines with black square) (A–D). Cell viability of the three different cell lines (MDA-MB-231, MCF-10A and 4T1) was also assessed after 24 h of stimulation (E) by MTT assay. The data are presented as percentage of cell viability relative to unstimulated cells (0 μM). Each point represents the average percentage ± SD. (n = 5) *p < 0.05 compared to unstimulated cells (0 μM).