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. 2018 Jan 31;8:1952. doi: 10.1038/s41598-018-20422-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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- DHA led to cell death in breast cancer cells. MDA-MB-231 (A) and 4T1 breast cells were stimulated with different concentrations of DHA for 24 h and cell death was assessed by labeling cells with annexin-V and propidium iodide followed by flow cytometry analysis. The percentage of necrotic, apoptotic or live cells is indicated. Data are representative of five independent experiments. UNS represents unstimulated cells. Each point represents the average percentage ± SD. (n = 5) *p < 0.05.