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. 2018 Jan 31;8:1952. doi: 10.1038/s41598-018-20422-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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- DHA triggered priming of inflammasome signaling pathway. Breast cancer MDA-MB-231 cells were stimulated with 50 or 100μM of DHA or AA (A) for 18 h and NFκB translocation towards to nucleus was analyzed by confocal microscopy (NF-κB in red and nuclei in blue) compared to unstimulated cells (UNS). MDA-MB-231 cells were also stimulated with 50, 100 or 200μM of DHA (B) for 6 h and expression of pro-caspase-1, pro-IL-1β and adaptor protein ASC was analyzed by western blotting and bands densitometry was performed using Image J software (C).