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. 2018 Jan 31;8:1949. doi: 10.1038/s41598-018-19458-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of the optimal number of reference genes for normalisation. Cells were cultured using RPMI 1640 medium containing 10% charcoal-stripped FBS for 24 hours prior to treatment with different DHT concentrations. The obtained Cq values were analysed and a pair-wise variation value (Vn/n + 1) generated by geNorm analysis across all prostate cancer cell lines with different DHT concentrations for mRNA reference genes (A), and for miRNA reference genes (B).