Table 1.

PCR efficiency for all primer pairs of reference genes and target genes. R² indicates correlation coefficient.

Gene	R2	Efficiency	Slope
18 S rRNA	0.998	0.906	−3.569
ACTB	0.997	1.065	−3.176
ALAS1	0.994	0.989	−3.346
GAPDH	0.997	0.924	−3.519
HPRT1	0.993	0.997	−3.330
K-ALPHA-1	0.998	0.987	−3.354
RPL13A	0.998	1.085	−3.134
SDHA	0.999	1.025	−3.263
TBP	0.995	1.025	−3.264
YWHAZ	0.997	1.009	−3.300
PCA3	0.993	0.979	−3.374
miR-16	0.999	0.917	−3.539
miR-130b	0.996	1.018	−3.280
miR-1225-3p	0.992	1.06	−3.186
miR-1228-3p	0.997	1.017	−3.283
RNU-6-2	0.998	0.982	−3.367
RNU-43	0.998	1.028	−3.257
miR-141	0.999	0.975	−3.383