Table 2.
Stability ranking of 10 mRNA reference genes and 6 npcRNA reference genes analysed by three algorithms across all treatments.
| geNorm | NormFinder | BestKeeper | Recommended comprehensive ranking | |||||
|---|---|---|---|---|---|---|---|---|
| Gene name | Ranking | Gene name | Ranking | Gene name | Ranking | Gene name | Geomean of ranking values | |
| mRNA gene | ACTB | 1 | ACTB | 1 | ACTB | 1 | ACTB | 1.00 |
| GAPDH | 1 | GAPDH | 2 | YWHAZ | 2 | GAPDH | 2.00 | |
| TBP | 3 | TBP | 3 | RPL13A | 3 | TBP | 3.98 | |
| HPRT1 | 4 | HPRT1 | 4 | GAPDH | 4 | RPL13A | 5.24 | |
| K-ALPHA-1 | 5 | SDHA | 5 | 18 S rRNA | 5 | HPRT1 | 5.43 | |
| SDHA | 6 | RPL13A | 6 | ALAS1 | 6 | YWHAZ | 5.45 | |
| 18 S rRNA | 7 | K-ALPHA-1 | 7 | TBP | 7 | SDHA | 6.21 | |
| RPL13A | 8 | 18 S rRNA | 8 | SDHA | 8 | 18 S rRNA | 6.54 | |
| YWHAZ | 9 | YWHAZ | 9 | K-ALPHA-1 | 9 | K-ALPHA-1 | 6.80 | |
| ALAS1 | 10 | ALAS1 | 10 | HPRT1 | 10 | ALAS1 | 8.43 | |
| npcRNA gene | miR-16 | 1 | miR-1225-3p | 1 | miR-16 | 1 | miR-16 | 1.26 |
| miR-1228-3p | 1 | miR-16 | 2 | miR-1228-3p | 2 | miR-1228-3p | 1.82 | |
| miR-1225-3p | 3 | miR-1228-3p | 3 | miR-1225-3p | 3 | miR-1225-3p | 2.08 | |
| miR-130b | 4 | miR-130b | 4 | RNU43 | 4 | miR-130b | 4.31 | |
| RNU43 | 5 | RNU43 | 5 | miR-130b | 5 | RNU43 | 4.64 | |
| RNU6-2 | 6 | RNU6-2 | 6 | RNU6-2 | 6 | RNU6-2 | 6.00 | |