Table 3.
Stability ranking of reference genes using geomean of ranking values analysed by three algorithms when prostate cells are treated with different DHT concentrations.
| Group | Gene | All treatments | 0 nM | 1 nM | 10 nM | 100 nM | 1000 nM | |
|---|---|---|---|---|---|---|---|---|
| mRNAgene | AR+celllines | ACTB | 1 | 1 | 3 | 2 | 5 | 1 |
| GAPDH | 2 | 4 | 6 | 3 | 4 | 5 | ||
| YWHAZ | 3 | 2 | 1 | 4 | 3 | 3 | ||
| SDHA | 4 | 5 | 2 | 1 | 2 | 4 | ||
| TBP | 5 | 3 | 4 | 6 | 6 | 6 | ||
| K-ALPHA-1 | 6 | 7 | 8 | 5 | 1 | 8 | ||
| 18 S rRNA | 7 | 6 | 5 | 9 | 9 | 9 | ||
| ALAS1 | 8 | 8 | 7 | 7 | 7 | 2 | ||
| RPL13A | 9 | 9 | 9 | 8 | 8 | 7 | ||
| HPRT1 | 10 | 10 | 10 | 10 | 10 | 10 | ||
| The other cell lines | ACTB | 1 | 1 | 5 | 1 | 2 | 1 | |
| HPRT1 | 2 | 4 | 1 | 4 | 3 | 2 | ||
| SDHA | 3 | 5 | 3 | 2 | 1 | 4 | ||
| RPL13A | 4 | 6 | 6 | 3 | 5 | 5 | ||
| TBP | 5 | 2 | 2 | 6 | 6 | 3 | ||
| ALAS1 | 6 | 3 | 4 | 7 | 8 | 7 | ||
| K-ALPHA-1 | 7 | 8 | 7 | 8 | 4 | 8 | ||
| YWHAZ | 8 | 7 | 8 | 9 | 9 | 6 | ||
| GAPDH | 9 | 9 | 9 | 5 | 10 | 10 | ||
| 18 S rRNA | 10 | 10 | 10 | 10 | 7 | 9 | ||
| npcRNAgene | AR+celllines | miR-16 | 1 | 2 | 1 | 2 | 3 | 1 |
| miR-1228-3p | 2 | 3 | 2 | 1 | 1 | 3 | ||
| miR-1225-3p | 3 | 1 | 4 | 3 | 2 | 2 | ||
| RNU43 | 4 | 5 | 5 | 5 | 4 | 6 | ||
| miR-130b | 5 | 6 | 3 | 4 | 5 | 5 | ||
| RNU6-2 | 6 | 4 | 6 | 6 | 6 | 4 | ||
| The other cell lines | RNU6-2 | 1 | 1 | 2 | 2 | 1 | 2 | |
| RNU43 | 2 | 2 | 1 | 1 | 3 | 1 | ||
| miR-1228-3p | 3 | 5 | 4 | 3 | 4 | 6 | ||
| miR-16 | 4 | 3 | 3 | 4 | 2 | 4 | ||
| miR-1225-3p | 5 | 4 | 6 | 6 | 5 | 3 | ||
| miR-130b | 6 | 6 | 5 | 5 | 6 | 5 |
Cell lines were divided into two groups: AR+ cell lines (LNCaP and 22RV1), and AR− and normal cell lines (DU145, PC-3 and RWPE-1).