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. 2018 Jan 31;8:2022. doi: 10.1038/s41598-018-20465-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene set enrichment analysis (GSEA) of microarray data in 22 dpp Mgat1 cKO germ cells. GSEA enrichment plots of three gene clusters that are enriched in Mgat1 cKO germ cells were (A) Gamete Generation, (B) Matzuk Spermatid Differentiation, and (C) Matzuk Spermatozoa Formation. Heat map of positively-enriched genes in Mgat1 cKO germ cells compared to control for (D) gamete generation (E) Matzuk spermatid differentiation and (F) Matzuk spermatozoa formation. *Genes involved in spermiogenesis that are up-regulated prematurely in Mgat1 cKO males at 22 dpp.