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. 2018 Jan 31;38(5):1085–1099. doi: 10.1523/JNEUROSCI.2127-17.2017

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Copyright © 2018 Kommaddi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 6. — Decreased ADF/cofilin phosphorylation and Drebrin levels in synaptosomes of APP/PS1 mice (see Fig. 6-1). A, Phosphorylation of cofilin (Ser3) is reduced in synaptosomes isolated from ADL APP/PS1 mice compared with age-matched WT mice. Statistical analysis: p-cofilin/cofilin, p = 0.0181, t = 2.631, df = 16; p-cofilin/tubulin, p = 0.0238, t = 2.498, df = 16; cofilin/tubulin, p = 0.7692, t = 0.3051, df = 16; n = 8–9 per group. B, C, ADF/cofilin-actin rods observed in primary neurons derived from APP/PS1 mice. Representative confocal images of primary cortical neurons from WT and APP/PS1 mice immunostained for actin (red) and cofilin (green). Arrows indicates ADF/cofilin-actin rods (B′). Insets show magnified images. Scale bar, 5 μm. D, Drebrin levels are reduced in synaptosomes isolated from ADL APP/PS1 mice compared with age-matched WT mice. Statistical analysis: Drebrin/tubulin, p = 0.0458, t = 2.146, df = 18; n = 10 per group. E, F, Arp2 and Arp3 protein levels are unaffected in synaptosomes isolated from ADL APP/PS1 mice compared with age-matched WT mice. Statistical analysis: Arp2/tubulin, p = 0.4899, t = 0.7090, df = 14; Arp3/tubulin, p = 0.9445, t = 0.07088, df = 14; n = 8 per group. Unpaired two-tailed t test was used. Asterisks indicate statistical significance for WT versus APP/PS1.