Figure 10.

Anxiety and depression-like behaviors examined in Tshz1 cKOs. A, B, In the elevated zero maze, no significant differences were observed between controls (n = 36) and cKOs (n = 29) in either the amount of time spent in the open quadrants of the maze (A) or in the number of entries into the open quadrants (B). C, D, During the open field test, Tshz1 cKOs (n = 18) traveled a greater total distance compared with controls (n = 25; C) and spent less time in the center of the chamber (D). E, In the FST, Tshz1 cKOs (n = 36) spent significantly more time floating compared with controls (n = 29), suggestive of a depression-like phenotype. Data are mean ± SEM. *p ≤ 0.05 (mixed linear factorial ANOVA). **p ≤ 0.01 (mixed linear factorial ANOVA). †††p ≤ 0.001 (two-tailed t test).