Figure 6.

Cs alters [Cl⁻]_i homeostasis in RT neurons. Gramicidin perforated patch recordings of muscimol-induced currents from RT (A) and VB (B) neurons at various command potentials, using a CsCl-based internal solution. The leak current has been subtracted from the representative traces. A1, Baseline E_GABA was more depolarized than in prior recordings with a KCl-based internal solution. Representative recording and current–voltage plot are shown. A2, Time series revealing that CsCl-based solutions mitigated the effect of VU0463271 application in E_GABA, relative to KCl-based recordings (Fig. 5). A3, Average change in E_GABA before and after 10 min of VU0463271 application. B1–B3, Similar measures examined in VB neurons, as described in A1–A3. VU0463271 application still shifted E_GABA in VB neurons, although the responses were blunted. *p < 0.05, **p < 0.01.