Figure 6.

The GnRH neuron model reproduces I_A, I_K, I_HVA, and I_LVA isolated in voltage-clamp experiments performed during negative feedback. Empirical data (gray) and model-simulated current (black) used in Hodgkin-Huxley modeling. Voltage protocols are located beneath the current responses in A, C, E, and G. Only those voltage steps used to estimate parameters for the simulated data are shown. A, I_LVA in response to a depolarizing voltage step in OVXE+ AM mice. Empirical data from Sun et al. (2010). B, Simulated and empirical I_LVA activation and inactivation curves from OVX+E mice during negative feedback. Empirical data from Zhang et al. (2009). C, Simulated and empirical I_HVA in response to depolarizing voltage steps (below current response) during negative feedback. Empirical data from Sun et al. (2010). D, Simulated and empirical I_HVA activation and inactivation curves determined from voltage-clamp experiments. Empirical data from Sun et al. (2010). E, Simulated and empirical I_K in response to depolarizing voltage steps during negative feedback. F, Simulated and empirical activation curves for I_K during negative feedback, determined from activation protocol in E. G, Simulated and empirical I_A during depolarizing voltage steps during negative feedback. H, Simulated and empirical activation and inactivation curves for I_A during negative feedback. Values from these fits populate Table 1, g_vc.