Table 4.
| Mean ± SEM of GnRH whole-cell passive properties from Figure 2 | ||||
|---|---|---|---|---|
| OVX AM | OVX PM | OVX+E AM | OVX+E PM | |
| Input resistance (mΩ) | 1019 ± 133 | 897.8 ± 39 | 955.6 ± 81 | 992.6 ± 82 |
| Capacitance (pF) | 15.5 ± 0.6 | 17.8 ± 0.8 | 16.9 ± 0.6 | 16.3 ± 1.0 |
| Series resistance (mΩ) | 11.8 ± 0.9 | 10.7 ± 0.5 | 11.4 ± 0.8 | 12.0 ± 1.0 |
| Holding current (pA) | −0.1 ± 2 | −6.4 ± 5 | 3.3 ± 3 | 0.3 ± 4 |
| Two-way ANOVA parameters for comparison of GnRH passive properties among groups: cells from OVX AM, OVX PM, OVX+E AM, OVX+E PM (Fig. 2) | ||||
| Estradiol | Time of day | Interaction | |
|---|---|---|---|
| Input resistance (mΩ) | F(1,41) = 0.03 | F(1,41) = 0.2 | F(1,41) = 0.7 |
| Capacitance (pF) | F(1,41) = 0.001 | F(1,41) = 1.1 | F(1,41) = 3.6 (p = 0.07) |
| Series resistance (mΩ) | F(1,41) = 0.3 | F(1,41) = 0.09 | F(1,41) = 1.0 |
| Holding current (pA) | F(1,41) = 2.0 | F(1,41) = 1.7 | F(1,41) = 0.2 |
| Mean ± SEM of GnRH whole-cell passive properties from Figure 3 | |||
| OVX AM | OVX PM | OVX+E AM | OVX+E PM | |
|---|---|---|---|---|
| Input resistance (mΩ) | ||||
| before kisspeptin | 1003 ± 111 | 970.8 ± 59 | 1097 ± 93 | 1283 ± 193 |
| during kisspeptin | 2061 ± 253 | 1378 ± 216 | 1711 ± 314 | 2302 ± 471 |
| Capacitance (pF) | ||||
| before kisspeptin | 17.3 ± 0.9 | 16.4 ± 0.9 | 16.0 ± 0.7 | 14.4 ± 1.2 |
| during kisspeptin | 18.0 ± 1.0 | 16.9 ± 0.9 | 16.9 ± 0.6 | 14.8 ± 1.3 |
| Series resistance (mΩ) | ||||
| before kisspeptin | 10.0 ± 0.5 | 10.5 ± 0.6 | 10.8 ± 0.7 | 10.5 ± 0.4 |
| during kisspeptin | 10.4 ± 0.8 | 10.5 ± 0.8 | 12.5 ± 1.3 | 10.8 ± 0.3 |
| Holding current (pA) | ||||
| before kisspeptin | 7.1 ± 3 | 0.8 ± 3 | 4.3 ± 3 | 2.3 ± 2 |
| during kisspeptin | −39 ± 6 | −39 ± 5 | −25 ± 5 | −18 ± 3 |
| Three-way ANOVA F(1,32) values for comparison of GnRH passive properties among groups: cells from OVX PM, OVX+E AM, and OVX+E PM (Fig. 3) | ||||
| E2 | AM/PM | Kisspeptin | E2 × kisspeptin | AM/PM × kisspeptin | E2 × AM/PM | E2 × kisspeptin × AM/PM | |
|---|---|---|---|---|---|---|---|
| Input resistance (mΩ) | 1.1 | 0.03 | 39*** | 0.3 | 0.4 | 3.4 (p = 0.07) | 3.6 (p = 0.07) |
| Capacitance (pF) | 3.1 (p = 0.09) | 2.4 | 43*** | 0.03 | 2.5 | 0.2 | 0.3 |
| Series resistance (mΩ) | 1.3 | 0.2 | 3.3 (p = 0.08) | 1.5 | 2.1 | 1.0 | 0.8 |
| Holding current (pA) | 5.2* | 0.01 | 283*** | 21*** | 3.4 (p = 0.08) | 0.7 | 0.2 |
| Mean ± SEM of GnRH whole-cell passive properties from Figure 4 | |||||||
| OVX PM | OVX+E AM | OVX+E PM | |
|---|---|---|---|
| Input resistance (mΩ) | 1194 ± 181 | 1314 ± 208 | 1531 ± 218 |
| Capacitance (pF) | 13.1 ± 1 | 14.0 ± 1 | 14.1 ± 0.8 |
| Series resistance (mΩ) | 11.9 ± 0.8 | 11.6 ± 0.9 | 11.1 ± 0.9 |
| Holding current (pA) | −3.3 ± 3 | −3.2 ± 4 | −10 ± 3.6 |
| One-way ANOVA parameters for comparison of GnRH passive properties among groups: cells from OVX PM, OVX+E AM, and OVX+E PM (Fig. 4) | |||
| Input resistance (mΩ) | KW statistic = 0.6 |
|---|---|
| Capacitance (pF) | F(2,22) = 0.4 |
| Series resistance (mΩ) | KW statistic = 0.6 |
| Holding current (pA) | F(2,22) = 1.3 |
*p < 0.05;
***p < 0.001.