Figure 1.
Primer setup used to amplify MHC class I exon 3 (MHCI) and MHC class II exon 2 (MHCII) in eight bluethroats. MHCI was sequenced in a single index run and in a dual index run on Ion Torrent. MHCII was additionally sequenced on Illumina MiSeq. The numbers are referring to the length of the respective parts of the primers