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. 2017 Dec 18;596(3):393–407. doi: 10.1113/JP275178

Figure 3. Inhibiting cAMP‐dependent intestinal anion secretion by pharmacological K+ channel blockade.

Figure 3

A–D and F, Ussing chamber traces of short circuit currents (I SC) as function of time obtained using distal colon of transgenic mice expressing the KCNE3‐D90N mutant subunit (A and C), a WT mouse (B), a Kcne3 −/− (D) and a Cftrtm1Eur mouse expressing the CFTR‐ΔF508 mutation (F). Details of compound additions are as in Fig. 1 except for the use of tetrapentylammonium (TPeA) added, unless otherwise indicated, basolaterally at 100 μm. Tissue resistances at the beginning and end of the experiment were 82 and 68 Ω cm2 in A, 98 and 70 Ω cm2 in B, 66 and 58 Ω cm2 in D, and 82 and 82 Ω cm2 in F. E, concentration dependence of the effect of basolateral TPeA on the cAMP‐activated anion secretion (normalized) measured using distal colon from Tg(Kcne3‐D90N)842cCECs mice expressing the KCNE3‐D90N subunit (means ± SD, n = 4).