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. 2018 Jan 31;8:1983. doi: 10.1038/s41598-018-20491-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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OP9-conditioned culture media delays differentiation of erythroid cells and increases expansion potential of cultures. CD34⁺ cells were cultured with OP9-conditioned culture media or control culture media for 19 days. (a) Comparison of the number of erythroid cells from day 0 to day 19 in cultures with OP9-conditioned media or control media (mean ± SD; n = 3, *p < 0.05, **p < 0.01; student’s t test). (b) Erythroid cells maintained in OP9-conditioned culture media or control culture media at day 16 and 19 stained with Leishman reagent and analyzed by light microscopy (scale bars 10 μm). Orange arrows indicate polychromatic erythroblasts, black arrows indicate orthochromatic erythroblast, red arrows indicate reticulocytes. (c) Morphological analysis of day 16 erythroid cells maintained in OP9 conditioned media and control media (mean ± SD; n = 3, *p < 0.05, **p < 0.01; student’s t test).