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. 2018 Jan 31;8:1964. doi: 10.1038/s41598-018-20306-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Rapid optical clearing using RTF. (a) Clearing steps of RTF and Clear^T2. The boxes in the diagram only indicate the step. (b) Whole embryos (E14.5) and neonatal (postnatal day 11, P11) whole-brain samples cleared with RTF and Clear^T2 overnight (transmission images). Grid size, 1.45 mm × 1.45 mm. (c) Adult brain slices (1-mm-thick) cleared with various clearing protocols. The outlines of the brain slices were drawn with dashed lines. Grid size, 1.45 mm × 1.45 mm. (d) Transmittance curves of the cleared mouse brain sections (1-mm-thick) (mean ± s.d., n = 6). (e) Normalized linear expansion of adult brain slices (1-mm-thick) after optical clearing (mean ± s.d., n = 6). Mann-Whitney U test was used to compare the difference of Clear^T2 with RTF. n.s., not significant. (f) Clearing of RTF is reversible with PBS. Grid size, 1.45 mm × 1.45 mm.