Fig. 6.

EM structure of shielded virus and crystal structure of hexon–scFv complex. a, b Comparison of EM structure of naked and shielded HAdV5. Color reflects distance to the core (white: <32 nm, red: 32–38 nm, yellow 41 nm, green 43 nm, cyan 46 nm, blue 48 nm). Trivalent shield proteins (green–blue) bind all over the capsid, resulting in a dense cover of viral capsid proteins (red–light green). c High-resolution crystal structure of scFv–hexon complex elucidates the atomic interactions. Both heavy (magenta) and light (cyan) chain of the scFv bind to the tower of a hexon monomer (three different shades of blue, one scFv displayed as surface, others as cartoon), formed mainly by HVR2 and HVR7. The structure also shows few interactions with HVR5. Importantly, all three epitopes in the trimeric hexon were occupied with three scFvs