Fig. 6.

Transfer of Treg cells rescues Xiap^−/− mice from lethal Candida albicans infection. a Increased expression of IFN-γ⁺ and IL-17⁺ cells in Xiap^−/− Treg cells upon Candida albicans infection. WT and Xiap^−/− female mice were intravenously infected with C. albicans (1 × 10⁵), and mice were killed at day 10. T cells were isolated from spleen, and the frequencies of CD4⁺Foxp3⁺, Foxp3⁺IFN-γ⁺ and Foxp3⁺IL-17⁺ cells were quantitated by intracellular staining. b Transfer of WT iTreg cells partially rescues Xiap^−/− mice from C. albicans-induced lethality. WT and Xiap^−/− female mice were intravenously administered with C. albicans (1 × 10⁵) and a group of Xiap^−/− mice also received WT iTreg cells (1 × 10⁶) at day 2. Survival of mice was monitored and is presented as a Kaplan–Meier survival curve (n = 7 for each group). **P < 0.01. c Reduced kidney inflammation in C. albicans-infected Xiap^−/− mice with WT iTreg cells transfer. Kidney was isolated from Xiap^−/− mice 10 days after C. albicans injection and infiltrated neutrophil contents were determined. d Reduced inflammatory cytokine production in C. albicans-infected Xiap^−/− mice into which WT iTreg cells had been transferred. Serum from mice was collected at the indicated time-points after C. albicans injection, and the levels of IL-6, TNF, MCP-1, and IL-12 were determined, n = 6. Values (a, d) are mean ± SD of samples. *P < 0.05, **P < 0.01, ***P < 0.001 for unpaired t-test