Figure 5.

Activity of TRPM4 channel is required for β‐catenin stability and GSK‐3β inhibitory phosphorylation. Overexpression of TRPM4 in T‐REx 293 (TREX) cells was induced by previous incubation with tetracycline 1 μg·mL⁻¹ (Tet). Cells were incubated for 2 h with the specific TRPM4 inhibitor 9‐phenanthrol at 10 μm or DMSO (vehicle) before protein extraction. (A, B) TRPM4 overexpression enhances β‐catenin protein levels, decreases its inhibitory phosphorylation, and increases the GSK‐3β S9 phosphorylation. The presence of 9‐phenanthrol reverts the effects of the TRPM4 overexpression on these intracellular proteins. (A) Representative western blots and the densitometries of at least three independent experiments (mean ± SEM) are shown. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001, multiple t‐test comparisons using t‐test with Welch correction.