Table I.
Primer sequences used for the determination of NOX4, p22phox, TNF-α, IL-1β and β-actin gene expression.
| Genes | Oligonucleotide primer sequences (5′-3′) | Product length (bp) |
|---|---|---|
| NOX4 | Forward, TGTGCCGAACACTCTTGGC | 136 |
| Reverse, ATATGCACGCCTGAGAAAATA | ||
| p22phox | Forward, TATTGTTGCAGGAGTGCTCA | 103 |
| Reverse, CACAGCGGTCAGGTACTTCT | ||
| TNF-α | Forward, GGCAGGTCTACTTTGGAGTC | |
| Reverse, GCAGGCAGTATCACTCATTG | 233 | |
| IL-1β | Forward, GCAGGCAGTATCACTCATTG | |
| Reverse, CACACCAGCAGGTTATCATC | 165 | |
| β-actin | Forward, GACTCCTATGTGGGTGACGA | 199 |
| Reverse, ACGGTTGGCCTTAGGGTTCA |
NOX4, NADPH oxidase 4; TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β.