Fig. 4. Tissue damage by TcdB.
A) H&E stained ceca. Tissues were collected from mice exposed to TcdB after 24 h and fixed in 4% formalin for H&E staining. B) Average histopathological scores of 4–5 mice with each TcdB treatment; C) MPO activity assay. A small portion of cecum from each mouse was collected and lysed for MPO assay. B1: TcdB 1 µg; B5: TcdB 5 µg; B25: TcdB 25 µg *: p < 0.05, vs PBS; **: p < 0.01, vs PBS; ***: p < 0.001, vs PBS.