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A) H&E stained ceca. Tissues were collected from mice exposed to mixtures of TcdA and TcdB after 24 h and fixed in 4% formalin for H&E staining. B) Average histopathological scores of 4–5 mice from each treatment. C) MPO activity assay. A small portion of cecum from each mouse was collected and lysed for MPO assay. A1: TcdA 1 µg; B5: TcdB 5 µg; A1B5: 1 µg TcdA + 5 µg TcdB; A2B2: 2 µg TcdA + 2 µg TcdB. *: p < 0.05, vs PBS; **: p < 0.01, vs PBS.
