Table 2.

Criteria for monitor physical conditions of mice^a.

Category	Scores
	0	1	2	3	4
Activity	Normal	Alert/slow moving	Lethargic/Shaky	Inactive unless Prodded	Not moving
Posture	Normal	Back slanted	Hunched	Hunched/nose down
Coat	Normal	Piloerection	Rough skin	Very ruffled/puff/ungroomed
Diarrhea	Normal	Hard to produce stool and looked Normal	Wet stained tail/red rectal/liquid stool
Eye/Nose	Normal	Squinted ½ closed	Squinted/discharge	Closed/discharge

Total possible score = 15; Normal = 0; Found dead = 15.

Mice with score equal or higher than 11 were euthanized.

^aClinical score = sum of all parameter scores.