Table 1.
Flow conditions for HF5 analyses. (Vc, longitudinal flow; Vx, cross/focus flow.)
| steps (versus) method | focus (ml min−1) | focus–injection (ml min−1) | elution (ml min−1) | elution–injection (ml min−1) |
|---|---|---|---|---|
| particle characterization | Vc = 0.35 | Vc = 0.35 | Vc = 0.35 | Vc = 0.35 |
| Vx = 0.80 | Vx = 0.80 | Vx = 0.1 | Vx = 0.0 | |
| time= 2 min | time = 3 min | time = 10 min | time = 3 min | |
| cationic Ag collection and fraction collection | Vc = 0.35 | Vc = 1.0 | Vc = 0.35 | Vc = 0.35 |
| Vx = 0.80 | Vx = 0.8 | Vx = 0.1 | Vx = 0.0 | |
| time = 0.5 min | time = 20 min | time = 10 min | time = 3 min |