Table 1.

Kinetics of daphnetin, 4-MDPN, 4-PDPN and 4-ADPN in HLC and S-COMT.

	Km (μM)		Vmax (nmol min−1 mg−1)		Clint (μl min−1 mg−1)
compound	HLC	S-COMT	HLC	S-COMT	HLC	S-COMT
daphnetin	0.21 ± 0.036	0.48 ± 0.071	0.11 ± 0.0034	81.4 ± 2.4	0.52	169.5
4-MDPN	0.20 ± 0.029	1.07 ± 0.10	0.14 ± 0.0039	165.7 ± 3.5	0.70	154.8
4-PDPN	0.089 ± 0.015	0.22 ± 0.034	0.17 ± 0.0047	93.3 ± 2.4	1.91	424.0
4-ADPN	7.52 ± 0.64	7.93 ± 1.31	0.13 ± 0.0038	247.0 ± 14.6	0.017	31.1