Table 3. Intra- and Interday Bias and Precision for All IMF Analogues Excluding the Isomeric IMFsa.
| bias
(%) |
precision (% CV) |
|||||
|---|---|---|---|---|---|---|
| analyte | expected concentration (ng mL–1) | mean (ng mL–1) | intraday (n = 3) | interday(n = 15) | intraday (n = 3) | interday (n = 15) |
| norfentanyl | 0.350 | 0.3652 ± 0.011 | 8.6 | 4.3 | 97.7 | 97.0 |
| 2.5 | 2.722 ± 0.17 | 18.0 | 8.9 | 94.3 | 93.5 | |
| 25 | 27.13 ± 0.67 | 10.0 | 8.5 | 98.3 | 97.4 | |
| furanyl norfentanyl | 0.350 | 0.3179 ± 0.023 | 17.0 | 9.2 | 86.0 | 92.5 |
| 2.5 | 2.351 ± 0.21 | 9.3 | 6.0 | 78.0 | 91.0 | |
| remifentanil acid | 0.350 | 0.3211 ± 0.052 | 14.0 | 8.3 | 72.0 | 83.0 |
| 2.5 | 2.327 ± 0.32 | 17.0 | 6.9 | 83.0 | 86.0 | |
| butyryl norfentanyl | 0.350 | 0.3630 ± 0.027 | 17.0 | 3.7 | 94.3 | 92.4 |
| 2.5 | 2.602 ± 0.20 | 18.0 | 4.1 | 94.1 | 92.0 | |
| remifentanil | 0.350 | 0.3353 ± 0.017 | 8.7 | 4.2 | 94.1 | 94.7 |
| 2.5 | 2.339 ± 0.17 | 12.0 | 6.4 | 94.0 | 92.6 | |
| acetyl fentanyl | 0.350 | 0.3478 ± 0.015 | 6.4 | 0.63 | 97.9 | 95.7 |
| 2.5 | 2.579 ± 0.21 | 17.0 | 3.2 | 93.7 | 91.6 | |
| alfentanil | 0.350 | 0.3210 ± 0.019 | 12.0 | 8.3 | 90.9 | 93.8 |
| 2.5 | 2.373 ± 0.15 | 9.4 | 5.1 | 92.9 | 93.6 | |
| AH-7921 | 0.350 | 0.2722 ± 0.11 | 65.0 | 22.0 | –32.0 | 59.0 |
| 2.5 | 1.761 ± 0.65 | 55.0 | 30.0 | 4.0 | 62.0 | |
| U-47700 | 0.350 | 0.3466 ± 0.038 | 13.0 | 0.97 | 87.0 | 89.0 |
| 2.5 | 2.292 ± 0.15 | 14.0 | 8.3 | 92.0 | 93.3 | |
| acetyl fentanyl4-methylphenethyl | 0.350 | 0.3628 ± 0.015 | 8.8 | 3.7 | 94.7 | 95.8 |
| 2.5 | 2.556 ± 0.15 | 9.0 | 2.2 | 92.7 | 93.9 | |
| acrylfentanyl | 0.350 | 0.3507 ± 0.014 | 5.0 | 0.20 | 94.9 | 96.0 |
| 2.5 | 2.502 ± 0.095 | 4.4 | 0.096 | 96.1 | 96.1 | |
| fentanyl | 0.350 | 0.3326 ± 0.015 | 13.0 | 5.0 | 98.2 | 95.3 |
| 2.5 | 2.458 ± 0.15 | 8.0 | 1.7 | 95.1 | 93.5 | |
| 25 | 24.77 ± 1.5 | 6.2 | 0.92 | 88.0 | 93.6 | |
| para-methoxyfentanyl | 0.350 | 0.3598 ± 0.018 | 9.1 | 2.8 | 92.5 | 94.7 |
| 2.5 | 2.533 ± 0.12 | 7.5 | 1.3 | 93.8 | 95.2 | |
| 4-ANPP | 0.350 | 0.3306 ± 0.043 | 20.0 | 5.5 | 76.0 | 87.0 |
| 2.5 | 2.373 ± 0.32 | 20.0 | 5.1 | 84.0 | 86.0 | |
| furanyl fentanyl | 0.350 | 0.3426 ± 0.015 | 4.4 | 2.1 | 94.6 | 95.5 |
| 2.5 | 2.487 ± 0.12 | 5.4 | 0.53 | 95.3 | 95.0 | |
| despropionyl para-fluorofentanyl | 0.350 | 0.2819 ± 0.044 | 23.0 | 19.0 | 71.0 | 84.0 |
| 2.5 | 2.006 ± 0.33 | 37.0 | 20.0 | 81.0 | 83.0 | |
| carfentanil | 0.350 | 0.3281 ± 0.011 | 9.7 | 6.3 | 96.6 | 96.5 |
| 2.5 | 2.366 ± 0.11 | 9.9 | 5.3 | 91.9 | 95.1 | |
| (±)-cis-3-methyl fentanyl | 0.350 | 0.3419 ± 0.030 | 18.0 | 2.3 | 94.1 | 91.0 |
| 2.5 | 2.418 ± 0.24 | 20.0 | 3.3 | 94.0 | 90.0 | |
| butyryl/isobutyryl fentanyl | 0.350 | 0.4488 ± 0.20 | 86.0 | 28.0 | 26.0 | 54.0 |
| 2.5 | 3.348 ± 1.4 | 95.0 | 34.0 | 31.0 | 55.0 | |
| para-fluorobutyryl/para-fluoroisobutyryl fentanyl | 0.350 | 0.3665 ± 0.042 | 19.0 | 4.7 | 92.1 | 88.0 |
| 2.5 | 2.709 ± 0.26 | 19.0 | 8.4 | 96.3 | 90.1 | |
| sufentanil | 0.350 | 0.2903 ± 0.035 | 30.0 | 17.0 | 87.0 | 88.0 |
| 2.5 | 2.074 ± 0.16 | 26.0 | 17.0 | 93.3 | 91.9 | |
| valeryl fentanyl | 0.350 | 0.3492 ± 0.015 | 6.0 | 0.23 | 95.3 | 95.7 |
| 2.5 | 2.480 ± 0.11 | 5.1 | 0.80 | 95.9 | 95.3 | |
a
Isomeric IMFs include butyryl, isobutyryl fentanyl, para-fluorobutyryl fentanyl, and FIBF. Underlined IMF analogues refer to successful quantitation that met acceptable criteria.