Characterization
of conjugation ratios of Oflx molecules with NPs
for three different sized Ag NPs using UV–vis absorption spectroscopy.
(A) UV–vis absorption spectra of (a) 2.4 ± 0.7 nm Ag NPs,
(b) AgMUNH2 NPs, and (c) AgMUNH-Oflx NPs show plasmonic
absorption peak wavelengths (λmax) of the NPs at
392, 413, and 416 nm, respectively. (B) UV–vis absorption spectra
of (a) 13.0 ± 3.1 nm Ag NPs, (b) AgMUNH2 NPs, and
(c) AgMUNH-Oflx NPs show plasmonic absorption λmax of the NPs at 394, 414, and 418 nm, respectively. (C) UV–vis
absorption spectra of (a) 92.6 ± 4.4 nm Ag NPs, (b) AgMUNH2 NPs, and (c) AgMUNH-Oflx NPs show plasmonic absorption λmax of the NPs at 450, 453, and 486 nm, respectively. Note
that signature absorption λmax of Oflx at 288 and
331 nm were observed only in (c) for the three sized nanocarriers,
showing the conjugation of Oflx with the NPs.