Table 1. Determination of the Conjugation Ratios of Ofloxacin (Oflx) Molecules per NP for Three Different Sized Drug Nanocarriers.
NP diameter (nm) | CNPsa (nM) | COflxb (μM) | ROflx/NPc |
---|---|---|---|
2.4 ± 0.7 | 50 | 43.2 | 8.6 × 102 |
13.0 ± 3.1 | 3.3 | 31.1 | 9.4 × 103 |
92.6 ± 4.4 | 0.030 | 19.4 | 6.5 × 105 |
Plasmonic absorbance of the drug nanocarriers (AgMUNH-Oflx NPs) at λmax of 416, 418, and 486 nm was used to determine the concentration of the 2.4 ± 0.7, 13.0 ± 3.1, and 92.6 ± 4.4 nm NPs, respectively.
COflx was determined by subtracting the UV–vis absorption spectra of AgMUNH2 NPs from that of AgMUNH-Oflx NPs and dividing the peak absorbance of the subtracted UV–vis spectra at 288 nm by the molar absorptivity (ε288nm) of Oflx, 7.8 × 103 M–1 cm–1.
Conjugation ratio of the number of Oflx molecules per NP was calculated by dividing the concentration of Oflx by the concentration of NPs for the same solution of AgMUNH-Oflx NPs for the 2.4 ± 0.7, 13.0 ± 3.1, and 92.6 ± 4.4 nm NPs, respectively.