Table 1. Determination of the Conjugation Ratios of Ofloxacin (Oflx) Molecules per NP for Three Different Sized Drug Nanocarriers.
| NP diameter (nm) | CNPsa (nM) | COflxb (μM) | ROflx/NPc |
|---|---|---|---|
| 2.4 ± 0.7 | 50 | 43.2 | 8.6 × 102 |
| 13.0 ± 3.1 | 3.3 | 31.1 | 9.4 × 103 |
| 92.6 ± 4.4 | 0.030 | 19.4 | 6.5 × 105 |
a
Plasmonic absorbance of the drug nanocarriers (AgMUNH-Oflx NPs) at λmax of 416, 418, and 486 nm was used to determine the concentration of the 2.4 ± 0.7, 13.0 ± 3.1, and 92.6 ± 4.4 nm NPs, respectively.
b
COflx was determined by subtracting the UV–vis absorption spectra of AgMUNH2 NPs from that of AgMUNH-Oflx NPs and dividing the peak absorbance of the subtracted UV–vis spectra at 288 nm by the molar absorptivity (ε288nm) of Oflx, 7.8 × 103 M–1 cm–1.
c
Conjugation ratio of the number of Oflx molecules per NP was calculated by dividing the concentration of Oflx by the concentration of NPs for the same solution of AgMUNH-Oflx NPs for the 2.4 ± 0.7, 13.0 ± 3.1, and 92.6 ± 4.4 nm NPs, respectively.