TABLE 5 .

Fourteen metabolic pathways were increased and three were reduced with increasing levels of A. nodosum (Tasco) in the diet^a

Trend between Con and treatment and predicted function	Con	1SW	3SW	5SW	P value
					Linear	Quadratic
Increased
Carbon fixation pathways in prokaryotesb	0.95 ± 0.01	1.07 ± 0.01	1.05 ± 0.01	1.01 ± 0.01	0.291	0.002
Histidine metabolism	0.60 ± 0.01	0.67 ± 0.01	0.66 ± 0.01	0.64 ± 0.02	0.223	<0.001
Phenylalanine metabolism	0.18 ± 0.00	0.20 ± 0.00	0.20 ± 0.00	0.20 ± 0.01	0.055	0.004
Tyrosine metabolism	0.31 ± 0.01	0.36 ± 0.01	0.35 ± 0.01	0.33 ± 0.01	0.735	0.003
Tropane, piperidine and pyridine alkaloid biosynthesis	0.11 ± 0.00	0.13 ± 0.00	0.13 ± 0.00	0.12 ± 0.01	0.214	0.008
Butanoate metabolism	0.56 ± 0.01	0.60 ± 0.01	0.58 ± 0.01	0.60 ± 0.01	0.027	0.491
Fatty acid biosynthesis	0.44 ± 0.02	0.50 ± 0.01	0.48 ± 0.02	0.47 ± 0.02	0.530	0.041
Fatty acid metabolism	0.18 ± 0.01	0.20 ± 0.00	0.19 ± 0.00	0.20 ± 0.00	0.005	0.326
Nicotinate and nicotinamide metabolism	0.47 ± 0.01	0.51 ± 0.01	0.79 ± 0.01	0.48 ± 0.02	0.932	0.036
Pantothenate and CoAc biosynthesis	0.65 ± 0.01	0.68 ± 0.01	0.69 ± 0.01	0.67 ± 0.02	0.244	0.028
Vitamin B6 metabolism	0.22 ± 0.01	0.24 ± 0.01	0.24 ± 0.00	0.24 ± 0.01	0.382	0.040
Prenyl transferases	0.32 ± 0.01	0.36 ± 0.01	0.36 ± 0.01	0.34 ± 0.02	0.410	0.040
Naphthalene degradation	0.12 ± 0.01	0.17 ± 0.01	0.15 ± 0.01	0.15 ± 0.01	0.189	0.047
Fructose and mannose metabolism	0.90 ± 0.02	0.98 ± 0.02	0.94 ± 0.01	0.91 ± 0.02	0.781	0.016
Decreased
Glycerophospholipid metabolism	0.52 ± 0.01	0.50 ± 0.01	0.50 ± 0.00	0.49 ± 0.01	0.026	0.811
Porphyrin and chlorophyll metabolism	0.91 ± 0.04	0.74 ± 0.03	0.81 ± 0.04	0.85 ± 0.03	0.931	0.021
Seleno compound metabolism	0.37 ± 0.01	0.34 ± 0.01	0.34 ± 0.01	0.35 ± 0.00	0.221	0.032

^aDM basis; n = 8 per diet. Con, control; 1SW, 1% DM-based Tasco; 3SW, 3% DM-based Tasco; 5SW, 5% DM-based Tasco. Data in columns 2 to 5 are average percentages and standard deviations.

^bMajor metabolic pathway.

^cCoA, coenzyme A.