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. 2018 Jan 9;16(1):19. doi: 10.3390/md16010019

Figure 4.

Figure 4

Pin-induced apoptosis is mediated through JNK and ERK pathway in HSCs. (A) HSC-T6 cells were treated with Pin (3–6 µg/mL) for 6 h. Phosphorylation of ERK and JNK were analyzed by immunoblot analysis using antibodies against p-JNK or p-ERK. Quantitation of the p-JNK/JNK and p-ERK/ERK ratio was shown; (B) HSC-T6 cells were pretreated with SP600125 (JNK inhibitor; 10 µM) or PD98059 (ERK inhibitor; 10 µM) for 1 h and then exposure to Pin (4 µg/mL) for 24 h. Cytotoxicity assay was monitored spectrophotometrically at 450 nm. All data are expressed as the mean ± S.E.M. (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the basal. ### p < 0.001 compared with the Pin alone.