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. 2018 Jan 16;16(1):31. doi: 10.3390/md16010031

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Penaeid shrimp Type II crustins form two distinct phylogenetic clades. The phylogenetic tree was constructed using the Neighbor-Joining method in MEGA 6. Bootstrap sampling was reiterated 1000 times. Sequences included in analyses were the following: (i) Type I crustins (“Carcinins”): the Pp-crustin from Portunus pelagicus (GenBank: JQ965930), the Sc-crustin from Scylla serrata (GenBank: HQ638025), the CrusSp from Scylla paramamosain (GenBank: EU161287), the CruHa1 from Hyas araneus (GenBank: EU921641), PtCrustin from Portunus trituberculatus (GenBank: FJ612106), the carcinin (11.5-kDa peptide) from Carcinus maenas (GenBank: AJ427538), the PlCrustin2 from Pacifastacus leniusculus (GenBank: EF523613), the Pc-crustin 2 from Procambarus clarkii (GenBank: GQ301202), the MjCruI-1 and MjCruI-2 from Marsupenaeus japonicus [11], and the carcininPm2 from Penaeus monodon [10]; (ii) Type IIa crustins (“Crustins”): the LvCrustin I (GenBank: AY488492), LvCrustin P (GenBank: AY488494), Crustin Lv1 (GenBank: AF430071) and Crustin Lv3 (GenBank: AF430073) from Litopenaeus vannamei (indicated by black circles, ●), the Crustin Ls1 (GenBank: AF430078), Crustin Ls2 (GenBank: AF430077) and Crustin Ls3 (GenBank: AF430079) from Litopenaeus setiferus, the CrusLsch (GenBank: EF182748) from Litopenaeus schmitti, the CrusFpau (GenBank: EF182747) from Farfantepenaeus paulensis, the CrusFbra (GenBank: EF601055) from Farfantepenaeus brasiliensis, the CrusFsub (GenBank: EF450744) from Farfantepenaeus subtilis, the Crustin Mj (GenBank: AB121741) from M. japonicus, the crustinPm1 (GenBank: FJ686014), crustinPm4 (GenBank: FJ686015), crustinPm5 (GenBank: FJ380049) and crustinPm6 (GenBank: GH717910) from P. monodon, the Fc-crus 2 (GenBank: FJ853147) from Fenneropenaeus chinensis and the PJC1 (GenBank: FJ797417), PJC2 (GenBank: FJ797418), PJC3 (GenBank: FJ797419), and PJC4 (GenBank: FJ797420) from Panulirus japonicus; (iii) Type IIb crustins (“Crustin-like”): the Crustin-like Lv (GenBank: JQ824114, FE049920 and FE049921) from L. vannamei (indicated by black squares, ■), the Crustin-like Mj from M. japonicus [16], the CruFc (GenBank: DQ097703) from F. chinensis, the Fi-crustin (GenBank: GQ469987) from Fenneropenaeus indicus and the Crustin-like Pm (GenBank: GQ334395, JX912161 and GU299808) and crustinPm7 (GenBank: EF654658) from P. monodon; (iv) Type III crustins (“Single WAP domain-containing proteins or SWD”): the LvSWD (GenBank: AY464465) and LvSWDi [40] from L. vannamei, the SWDPm1 (GenBank: EU623979) and SWDPm2 (GenBank: EU623980) from P. monodon, the FcSWD (GenBank: EF216349) from F. chinensis and the MjSWD (GenBank: AU176270) from M. japonicus; (v) Type IV crustins (“Double WAP domain-containing proteins or DWD”): the LvSLPI (GenBank: EF467169) from L. vannamei, the PmDWD (GenBank: BI784457) from P. monodon, the FcDWD (GenBank: GQ303571) from F. chinensis, and the MjDWD (GenBank: EU095018) from M. japonicus.