Table 1.
Gene | Forward Primer (5′-3′) | Reverse Primer (5′-3′) | Amplicon |
---|---|---|---|
Primers for Tissue Distribution Analysis (RT-PCR) | |||
β-actin | TAATCCACATCTGCTGGAAGGTGG | TCACCAACTGGGATGACATGG | 846 bp |
Crustin Lv | CGAACCAGAGACACCTGTTG | CAGCACACTTGTAGTCGTTG | 113 bp |
Crustin-like Lv | GCAGGATAAAGACAAGGC | GTAATTGCAGTTGAATCCGCC | 199 bp |
Primers for Quantitative Analysis of Gene Expression (RT-qPCR) | |||
LvEF1α | TGGCTGTGAACAAGATGGACA | TTGTAGCCCACCTTCTTGACG | 103 bp |
LvRpS6 | AGCAGATACCCTTGGTGAAG | GATGCAACCACGGACTGAC | 193 bp |
LvL40 | GAGAATGTGAAGGCCAAGATC | TCAGAGAGAGTGCGACCATC | 104 bp |
LvRpS3A | GGCTTGCTATGGTGTGCTCC | TCATGCTCTTGGCTCGCTG | 101 bp |
β-actin | CCACGAGACCACCTACAAC | AGCGAGGGCAGTGATTTC | 142 bp |
Crustin Lv | CGAACCAGAGACACCTGTTG | CAGCACACTTGTAGTCGTTG | 113 bp |
Crustin-like Lv | GCAGGATAAAGACAAGGC | GTAATTGCAGTTGAATCCGCC | 199 bp |