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. 2018 Jan 16;16(1):31. doi: 10.3390/md16010031

Table 1.

Nucleotide sequences of primers used in this study.

Gene Forward Primer (5′-3′) Reverse Primer (5′-3′) Amplicon
Primers for Tissue Distribution Analysis (RT-PCR)
β-actin TAATCCACATCTGCTGGAAGGTGG TCACCAACTGGGATGACATGG 846 bp
Crustin Lv CGAACCAGAGACACCTGTTG CAGCACACTTGTAGTCGTTG 113 bp
Crustin-like Lv GCAGGATAAAGACAAGGC GTAATTGCAGTTGAATCCGCC 199 bp
Primers for Quantitative Analysis of Gene Expression (RT-qPCR)
LvEF1α TGGCTGTGAACAAGATGGACA TTGTAGCCCACCTTCTTGACG 103 bp
LvRpS6 AGCAGATACCCTTGGTGAAG GATGCAACCACGGACTGAC 193 bp
LvL40 GAGAATGTGAAGGCCAAGATC TCAGAGAGAGTGCGACCATC 104 bp
LvRpS3A GGCTTGCTATGGTGTGCTCC TCATGCTCTTGGCTCGCTG 101 bp
β-actin CCACGAGACCACCTACAAC AGCGAGGGCAGTGATTTC 142 bp
Crustin Lv CGAACCAGAGACACCTGTTG CAGCACACTTGTAGTCGTTG 113 bp
Crustin-like Lv GCAGGATAAAGACAAGGC GTAATTGCAGTTGAATCCGCC 199 bp