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U-0126 monoethanolate (U-0126) (10 μM) counteracted AFM1 (12 μM) and OTA (20 μM)-induced p44/42 mitogen-activated protein kinase (MAPK) (extracellular regulated protein kinases 1/2, [ERK] 1/2). Differentiated Caco-2 cells were either pretreated with U-0126 or complete cultivation medium for 1 h before addition of individual and combined AFM1 and OTA for 30 min. Phosphorylation of p44/42 MAPK was determined by immunoblotting.
