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. 2017 Dec 29;10(1):22. doi: 10.3390/toxins10010022

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunostaining for α-synuclein: Lewy body inclusions (indicated by arrows) in the substantia nigra pars compacta of a 120-day old female rat exposed to BMAA on PND3 (A) and a 120-day old male rat exposed to 400 mg/kg BMAA on PND3 (B,C). The percentage alpha-synuclein positive burden, defined as the percentage neurons in the substantia nigra pars compacta that harbor Lewy body inclusions, is given for all male (n = 5) (D) and female (n = 5) (E) exposure groups. * indicates significant difference to the control (p < 0.05). Scale bars correspond to 25 μm (A) and 10 μm (B,C).