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. 2018 Jan 7;10(1):34. doi: 10.3390/toxins10010034

Figure 2.

Figure 2

Interaction of Az with muscle and neuronal nAChRs. Inhibitory curves of Az action on ACh (30 and 10 μM)-evoked intracellular calcium concentration ([Ca2+]i) rises in neuroblastoma Neuro2a cells expressing (a) muscle α1β1εδ and (b) α7 nAChRs, respectively. (c) Representative nicotine (Nic)-induced current traces through α4β2 nAChR and (d) dose-response curve of [Ca2+]i amplitude rise in neuroblastoma SH-SY5Y cells expressing α3-containing nAChRs in response to different concentrations of Nic. There are no inhibitory effects of Az on Nic-evoked (c) ion currents and (f) calcium responses mediated by α4β2 and α3-containing nAChRs, respectively (p > 0.05, Mann–Whitney U test). (e) Inhibitory curve of α-conotoxin MII action on Nic (100 μM)-evoked [Ca2+]i rise in SH-SY5Y cells expressing α3-containing nAChRs. Each point represents data obtained from 4 independent experiments (mean ± SEM).